Determining antibiotic concentration in human blood provides useful pharmacokinetic\ninformation. Commonly used methods such as ELISA require a long time to obtain results and thus\ncannot be applied when information is needed immediately. In this study, a novel antibody-based\nlateral flow technique was developed for tetracycline detection in human serum. Contrary to tests\ndeveloped to analyze food samples, the features of work with serum as analyzed probe were studied\nfor the first time here. The application of labeled and unlabeled specific antibodies was compared.\nFor this purpose, specific and anti-species antibodies were labeled with gold nanoparticles and used\nfor antigenâ??antibody interaction on the membrane surface with observed staining in the test zone.\nFor both schemes, optimal conditions were established to provide the best sensitivity. The developed\nassay has a limit of visual detection as low as 35 and 11 ng/mL for the direct and indirect labeled\nantibodies, respectively. The limit of instrumental detection is from 0.4 to 3.5 ng/mL for diluted and\nundiluted sera. The use of indirect antibody labeling showed a small increase in sensitivity compared\nto traditional direct antibody labeling. The developed method showed no cross-reactivity with\nantibiotics of other classes. The method was used to test samples of serum. The results showed high\ncorrelation with the data obtained by ELISA (R2 = 0.98968). The assay provides a quick assessment\nof the amount of antibiotics in the blood and keeps them under control throughout the duration\nof therapy.
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